Thanks for a very interesting & informative paper.
Could you please comment on the following

>Rainbow trout effluent was pumped with peristaltic pumps
>(model no. 7520-35 Cole Parmer Instrument Co., Chicago, IL)
>at a constant flow rate of 250 and 300 ml/min for basil and
>lettuce, respectively.

Is there any reason other than accurate flow rates for analysis that you
used peristaltic pumps ? It would appear to be excessively expensive for a
commercial system.

What were the reasons for selecting Lettuce & Basil for the project ?

With a 72 foot trough was there any effect from oxygen depletion in the
effluent ?
Was the BOD measured after microfiltering ? and could high BOD cause
problems considering the effluent is only in the system for  around 150
minutes (assuming 2mm film)?

Regards
John Harris
Dalwallinu, Australia